CRISPRi

A recent CRISPR study contradicted years of RNA interference research on a well-studied cancer drug target. But is it the last nail in the coffin for RNAi as a screening tool? 

Using a modified CRISPR approach, scientists shed light on the diversity and function of 499 long noncoding RNAs.

Two independent groups demonstrate the utility of CRISPR-based techniques to identify regulatory elements that govern disease-linked genes. 

Tips on how to surmount the challenges of working with CRISPR to manipulate genes in human stems cells to study their function in specific diseases or to correct genetic defects in patient cells.

Scientists identify a novel CRISPR system that zeroes in on single-stranded RNA.  

Ever-improving CRISPR-based tools are already ripe for large-scale genetic screens.

A variation of the gene-editing technique can more precisely and efficiently downregulate the expression of target genes than traditional CRISPR/Cas9.

Newer CRISPR tools for manipulating transcription will help unlock noncoding RNA’s many roles.

A newcomer’s guide to the hottest gene-editing tool on the block